Figure 2

Functional analyses of uc.4. (a) Optical and fluorescence microscopy (× 100) were used to determine the lentivirus transfection efficiency of the uc.4 overexpression and control vectors via GFP expression cells after 2 weeks of puromycin selection. (b) uc.4 expression levels in P19 cells were significantly higher than in the NC groups. ***P<0.001, Student’s t-test. (c) Proliferation was analyzed using CCK-8 at 0, 24, 48, 72 and 96 h. The OD450 value represents the proliferation capacity. (d) The cell cycle was analyzed using flow cytometry. (e) Flow cytometry was performed to analyze apoptosis. Data are presented as the means±s.d. of three independent experiments. GFP, green fluorescent protein; NC, negative control.