Figure 5
From: Sleep deprivation disrupts the lacrimal system and induces dry eye disease
Morphological change and inflammation of the lacrimal gland after sleep deprivation (SD). Lacrimal gland size (a) and weight (b) both markedly increased after SD for 2 days, and these increases became more prominent at day 10 (n=6/group, ***P<0.001). (c) Hematoxylin and eosin (H&E) staining showed enlarged lacrimal gland acini after SD for 2 and 10 days. High magnification pictures in the lower row show vesicle accumulation in acini cells, which was more prominent at day 10 compared with day 2. The scale bars represent 100 μm. (d) Immunostaining of lacrimal gland tissues showed no obvious CD45-positive cell infiltration after SD for different durations. The scale bar represents 100 μm. (e) RNA-seq results showed that the cell proliferation-related genes Ccnb1, Cdkn3, Cdca8, Bub1, Bub1b, Cdk1, Mad2l1, Ccnb2, Mli67, Cdkn2c and Cdk14 were strongly upregulated at 2 days of SD but returned to normal levels at 10 days of SD. (f) The cell apoptosis-related genes Bax, Trp53 and Fas obviously increased at 10 days of SD. The data were transferred to the Z-score log 2-Fpkm by each row and clustered based on the Euclidean distance (n=3/group).
