Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Intrahippocampal injection of a lentiviral vector expressing neurogranin enhances cognitive function in 5XFAD mice

Figure 2

Hippocampal injection of LentiM1.2-Ng (LV-Ng) compensated for the reduction in neurogranin (Ng) expression in the hippocampus of 5XFAD mice. (a) Experimental scheme for the behavioral studies and stereotaxic surgery. Behavioral tests were performed 9 weeks after surgery, and animals were killed 10 weeks after stereotaxic injection. (b) Representative images of Ng expression in the dentate gyrus of the hippocampus. LentiM1.2 (empty vector) (LV-control)-injected 5XFAD mice showed less immunoreactivity compared with their wild-type littermates. The LV-Ng-injected groups exhibited elevated Ng immunoreactivity in the brains of 5XFAD and wild-type mice. Scale bars=50 μm. (c) Immunoreactivity of Ng was statistically analyzed by one-way analysis of variance (ANOVA) followed by Fisher’s least significant difference (LSD) test (F(3, 60)=9.272, P=0.0129). ***P<0.001.

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