Figure 3

Mice with enhanced neurogranin (Ng) expression had fewer cognitive dysfunctions than 5XFAD mice. (a) One-way analysis of variance (ANOVA) followed by Fisher’s least significant difference (LSD) test indicated that 5XFAD mice exhibited significantly fewer spontaneous alterations compared to their wild-type littermates (shown by **), and LentiM1.2-Ng (LV-Ng)-injected 5XFAD mice (n=7–8) exhibited significantly more spontaneous alternations compared to LentiM1.2 (empty vector) (LV-control)-injected 5XFAD mice (shown by *) in the Y-maze test (F(3, 25)=3.679, P=0.025). No significant difference in spontaneous alternations was noted between the LV-control- and LV-Ng-injected wild-type littermate mice. (b) The number of total arm entries was not significantly different between the groups. (c) A significant difference between the familiar object and novel object explorations times was found for LV-Ng-injected 5XFAD mice. (d) The total exploration time indicated that LV-Ng injection did not significantly increase the exploration time in wild-type and 5XFAD mice compared with that in LV-control-injected mice. Error bars indicate the standard error of the mean. *P<0.05, *P<0.01 and ***P<0.001.