Figure 1

MiR-708-3p expression decreased during fibrosis in vivo and in vitro. (a) MiR-708-3p expression diminished in the lung tissues treated with BLM for 0, 7, 14, 21 and 28 days, as indicated by qRT-PCR. The miR-708-3p expression was normalized to the sham group. (b) α-SMA expression increased in the BLM group relative to that in the sham group. (c) Pearson correlation coefficient clarified that miR-708-3p inversely correlated with α-SMA in vivo. (d) The qRT-PCR results showed that miR-708-3p expression decreased in the TGF-β1-treated A549 and MRC-5 cells. (e) α-SMA increased in the TGF-β1-treated A549 and MRC-5 cells. (f) MiR-708-3p expression levels were inversely correlated with α-SMA in vitro. (g) The qRT-PCR results showed that pri-miR-708 and pre-miR-708 expression decreased in the TGF-β1-treated A549 and MRC-5 cells. (h) MiR-708 promoter activity decreased in A549 cells treated with TGF-β1 for 72 h. The pGL3-basic indicates the negative control. (i) MiR-708-5p expression decreased in A549 cells and increased in MRC-5 cells treated with TGF-β1. Each bar represents the mean±s.d.; n=6. *P<0.05, **P<0.01.