Figure 3

Identification of the miR-708-3p-targeted gene. (a) Sequences of miR-708-3p and the potential miR-708-3p binding site at the 3′UTR of ADAM17. The nucleotides mutated in the ADAM17-3′UTR mutant are also shown. The luciferase activity of WT 3′UTR-ADAM17 significantly decreased in the cells transfected with miR-708-3p mimics, whereas the miR-708-3p mimics could not inhibit the luciferase activity of MU 3′UTR-ADAM17. (b) The miR-708-3p mimic inhibited ADAM17 expression in A549 and MRC-5 cells. (c) The miR-708-3p inhibitor promoted ADAM17 expression in A549 and MRC-5 cells. (d) Pearson correlation coefficient showed that miR-708-3p inversely correlated with ADAM17. (e–h) Rescue experiments (miR-708-3p mimic+ADAM17 overexpression) confirmed ADAM17 as the target of miR-708-3p in A549 and MRC-5 cells, respectively. Each bar represents the mean±s.d.; n=6, *P< 0.05, **P<0.01.