Figure 6
From: Upregulation and biological function of transmembrane protein 119 in osteosarcoma
Silencing of TMEM119 inhibits the migration and invasion of osteosarcoma cells. (a, b) Migration and invasion assays were performed on siRNA-transfected U2OS (a) and MG63 cells (b). Magnification: × 200, scale bars: 100 μm. (c) Protein levels of metastasis-related proteins (MMP2, Twist1, ZEB1, E-cadherin, N-cadherin, vimentin and α-SMA) in osteosarcoma cell lines were detected by western blotting. (d) Protein levels of TGF-β pathway-related factors (BMP2, BMP7 and TGF-β) in U2OS and MG63 cells were detected by western blotting. The experiments were repeated at least three times with similar results. **P<0.01, ***P<0.001.
