Figure 8

TGF-β inhibitor or BMP inhibitor impairs the effects of TMEM119 overexpression on migration and invasion. (a, b) Saos2 cells were infected with vector of the TRMEM119 lentivirus, and migration and invasion assays were performed in a Boyden chamber containing either DMSO (vehicle) or 10 μM SB431542 (SB; Sigma). Magnification: × 200, scale bars: 100 μm. (c) Phosphorylation of Smad2/3 was determined by western blotting. (d, e) Saos2 cells infected with vector of the TRMEM119 lentivirus, and migration and invasion assays were performed in a Boyden chamber containing either DMSO (vehicle) or 5 μM dorsomorphin (DM; Sigma). Magnification: × 200, scale bars: 100 μm. (f) Phosphorylation of Smad1/5/8 was determined by western blotting. The experiments were repeated at least three times with similar results. *P<0.05, **P<0.01, ***P<0.001 (n=3).