Figure 4
From: A FKBP5 mutation is associated with Paget’s disease of bone and enhances osteoclastogenesis
Expression of osteoclast-specific molecules and transcription factors in mice BMMs. (a) Relative expression levels of NFATC1 mRNA. NFACT1 mRNA levels significantly increased following induction by RANKL in both wild-type and FKBP51V55L mutant BMMs. NFATC1 mRNA was higher in FKBP51V55L mutant BMMs than in wild-type BMMs after incubation of the cells with RANKL for 2 days. (b) Relative expression levels of TRAP mRNA. TRAP mRNA expression significantly increased in BMMs after induction by RANKL, and significantly more TRAP was detected in FKBP51V55L mutant BMMs than in wild-type BMMs. (c) Relative expression levels of OSCAR mRNA. OSCAR mRNA expression levels in BMMs were elevated several thousand-fold after induction by RANKL. Significantly more OSCAR mRNA expression was detected in FKBP51V55L mutant BMMs than in wild type. (d) Agarose gel electrophoresis image of one representative result of the real time RT-PCR experiment. (e) One representative western blotting result of c-fos expression in wild-type and FKBP51V55L mutant BMMs in response to RANKL exposure for the indicated time. (f) One representative western blotting result of NFATC1 expression in wild-type and FKBP51V55L mutant BMMs in response to RANKL exposure for the indicated number of days. All data shown are representative of three independent experiments. *P<0.05. OSCAR, osteoclast-associated receptor; TRAP, tartrate-resistant acid phosphatase.
