Figure 5
From: A FKBP5 mutation is associated with Paget’s disease of bone and enhances osteoclastogenesis
Detection of osteoclastogenesis-related signaling pathways in mouse BMMs. (a) A representative western blotting result used for signaling pathways detection. Akt phosphorylation is increased in FKBP51V55L mutant BMMs in response to RANKL; however, IκB degradation and ERK phosphorylation show no significant differences in wild-type and mutant BMMs. (b) LC3 expression in BMMs during osteoclast differentiation. LC3II expression in FKBP51V55L mutant and wild-type BMMs induced by RANKL (100 ng ml−1) and M-CSF (50 ng ml−1) is nearly the same. All results are representative of at least two biological replicates.
