Table 2 Primers used for qRT-PCR

From: Elevated TRAF4 expression impaired LPS-induced autophagy in mesenchymal stem cells from ankylosing spondylitis patients

Gene	Accession No.	Forward primer (5′–3′)	Reverse primer (5′–3′)	Product size (bp)
A20	NM_006290	TCCTCAGGCTTTGTATTTGAGC	TGTGTATCGGTGCATGGTTTTA	124
AP1	NM_002228	TCCAAGTGCCGAAAAAGGAAG	CGAGTTCTGAGCTTTCAAGGT	78
ATG5	NM_004849	AAAGATGTGCTTCGAGATGTGT	CACTTTGTCAGTTACCAACGTCA	144
ATG7	NM_001144912	ATGATCCCTGTAACTTAGCCCA	CACGGAAGCAAACAACTTCAAC	114
ATG12	NM_004707	CTGCTGGCGACACCAAGAAA	CGTGTTCGCTCTACTGCCC	97
ATG16L1	NM_198890	ATGCGCGGATTGTCTCAGG	GTCCACTCATTACACATTGCTCT	138
BCL2	NM_000657	GGTGGGGTCATGTGTGTGG	CGGTTCAGGTACTCAGTCATCC	89
BECN1	NM_003766	GGTGTCTCTCGCAGATTCATC	TCAGTCTTCGGCTGAGGTTCT	121
GAPDH	NM_001256799	GGAGCGAGATCCCTCCAAAAT	GGCTGTTGTCATACTTCTCATGG	197
IKKα	NM_001278	GGCTTCGGGAACGTCTGTC	TTTGGTACTTAGCTCTAGGCGA	87
IKKβ	NM_001556	GGAAGTACCTGAACCAGTTTGAG	GCAGGACGATGTTTTCTGGCT	147
IKKɛ	NM_001193322	TGCCTGAGGATGAGTTCCTG	CGATGCACAATGCCGTTCT	82
IKKγ	NM_003639	AAGAGCCAACTGTGTGAGATG	TTCGCCCAGTACGTCCTGA	69
IRAK4	NM_001145258	CCTGACTCCTCAAGTCCAGAA	ACAGAAATGGGTCGTTCATCAAA	119
IRAKM	NM_007199	CTGCGGGATCTCCTTAGAGAA	GCAGAGAAATTCCGAGGGCA	101
IRF3	NM_001197128	AGAGGCTCGTGATGGTCAAG	AGGTCCACAGTATTCTCCAG	100
IRF4	NM_002460	GCTGATCGACCAGATCGACAG	CGGTTGTAGTCCTGCTTGC	111
IRF5	NM_001098630	GGGCTTCAATGGGTCAACG	GCCTTCGGTGTATTTCCCTG	138
IRF7	NM_004029	GCTGGACGTGACCATCATGTA	GGGCCGTATAGGAACGTGC	84
IRF8	NM_002163	ATGTGTGACCGGAATGGTGG	AGTCCTGGATACATGCTACTGTC	77
IRF9	NM_006084	GCCCTACAAGGTGTATCATTG	TGCTGTCGCTTTGATGGTACT	84
MyD88	NM_001172569	GGCTGCTCTCAACATGCGA	CTGTGTCCGCACGTTCAAGA	61
SOCS1	NM_003745	CACGCACTTCCGCACATTC	TAAGGGCGAAAAAGCAGTTCC	191
RIP1	NM_003804	GGGAAGGTGTCTCTGTGTTTC	CCTCGTTGTGCTCAATGCAG	91
TAK1	NM_003298	CCTCTGGCCCATTGAGTGTTT	GTCCACTGCGGTGACTATCTG	98
TBK1	NM_013254	TGGGTGGAATGAATCATCTACGA	GCTGCACCAAAATCTGTGAGT	124
TIRAP	NM_148910	GACCCCTGGTGCAAGTACC	CGACGTAGTACATGAATCGGAG	133
TRAF1	NM_001190945	TCCTGTGGAAGATCACCAATGT	GCAGGCACAACTTGTAGCC	117
TRAF3	NM_003300	GCGTGTCAAGAGAGCATCGTT	GCAGATGTCCCAGCATTAACT	133
TRAF4	NM_004295	TATTGGGCCTGCCTATCCG	CAAAACTCGCACTTGAGGGG	205
TRAF6	NM_145803	ATGCGGCCATAGGTTCTGC	TCCTCAAGATGTCTCAGTTCCATD	198

Abbreviations: A20, tumor necrosis factor-alpha induced protein 3; AP1, activator protein 1; ATG5, autophagy-related 5; ATG7, autophagy-related 7; ATG12, autophagy-related 12; ATG16L1, autophagy-related 16 like 1; BECN1, beclin-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IKKα, inhibitor of kappaB kinase alpha; IKKβ, inhibitor of kappaB kinase beta; IKKɛ, inhibitor of kappaB kinase epsilon; IKKγ, inhibitor of kappaB kinase gamma; IRAK4, interleukin-1 receptor-associated kinase 4; IRAKM, interleukin-1 receptor-associated kinase M; IRF3, interferon regulatory factor 3; IRF4, interferon regulatory factor 4; IRF5, interferon regulatory factor 5; IRF7, interferon regulatory factor 7; IRF8, interferon regulatory factor 8; IRF9, interferon regulatory factor 9; MyD88, myeloid differentiation primary response 88; SOCS1, suppressor of cytokine signaling 1; RIP1, rieske iron–sulfur polypeptide 1; TAK1, transforming growth factor-beta activated kinase 1; TBK1, TANK binding kinase 1; TIRAP, toll/interleukin-1 receptor-like protein domain-containing adaptor protein; TRAF1, tumor necrosis factor receptor-associated factor 1; TRAF3, tumor necrosis factor receptor-associated factor 3; TRAF4, tumor necrosis factor receptor-associated factor 4; TRAF6, tumor necrosis factor receptor-associated factor 6.

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Table 2 Primers used for qRT-PCR

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