Table 1 Primer sequences for qRT–PCR
Gene | Sequence |
|---|---|
miR-214 | |
upstream | 5′-AGCATAATACAGCAGGCACAGAC-3′ |
downstream | 5′-AAAGGTTGTTCTCCACTCTCTCAC-3′ |
Mfn2 | |
upstream | 5′-ATGTCCCTGCTCTTCTCTCGATGC-3′ |
downstream | 5′-TCTGCTGGGCTGCAGGTACTGGT-3′ |
E-cadherin | |
upstream | 5′-CCCACCACGTACAAGGGTC-3′ |
downstream | 5′-ATGCCATCGTTGTTCACTGGA-3′ |
N-cadherin | |
upstream | 5′-TGAAGTCCCCAATGTCTCCA-3′ |
downstream | 5′-GCATCATCATCCTGCTTATCC-3′ |
Fibronectin | |
upstream | 5′-CCCCATTCCAGGACACTTCTG-3′ |
downstream | 5′-GCCCACGGTAACAACCTCTT-3′ |
Twist1 | |
upstream | 5′-GAGGCGCCCCGCTCTTCTCCTCTG-3′ |
downstream | 5′-CGTCTGAAGAACGGCGCGAA-3′ |
Snail | |
upstream | 5′-GAATTCATGCCGCGCTCTTTCCTCGTCAG-3′ |
downstream | 5′-CTCGAGCCTCGAGGGTCAGCGGGGACATC-3′ |
Vimentin | |
upstream | 5′-AGAACTTTGCCGTTGAAGCTG-3′ |
downstream | 5′-CCAGAGGGAGTGAATCCAGATTA-3′ |
ZO-1 | |
upstream | 5′-CAACATACAGTGACGCTTCACA-3′ |
downstream | 5′-GACGTTTCCCCACTCTGAAAA-3′ |
GAPDH | |
upstream | 5′-GGTCACCAGGGCTGCTTTTA-3′ |
downstream | 5′-GAGGGATCTCGCTCCTGGA-3′ |
