Figure 3
MARCO polymorphisms and variation in mRNA expression or cytokine production from healthy subjects. (a) mRNA was isolated from monocytes stimulated with Media, LPS at 100 ng ml−1 or M. tuberculosis whole-cell lysate at 5 μg ml−1. MARCO mRNA expression was measured and normalizes to glyceraldehyde-3-phosphate dehydrogenase. (b) Association of MARCO mRNA expression from cells stimulated with M. tuberculosis whole-cell lysate was analyzed with SNPs in MARCO: rs2278589 (4 AA, 12 AG, 15 GG), P=0.068 and rs6751745 (3 AA, 13 AG, 15 GG), P=0.039. (c) Cytokines were measured from monocytes stimulated with media, TDM at 100 μg ml−1 or M. tuberculosis whole-cell lysate at 25 μg ml−1. Cytokines from cells stimulated with TDM (d) or M. tuberculosis whole-cell lysate (e) were analyzed with SNP rs2278589 (4 AA, 12 AG, 15 GG). Cytokines from cells stimulated with TDM (f) or M. tuberculosis whole-cell lysate (g) were analyzed with SNP rs6751745 (3 AA, 13 AG, 15 GG). Data were collected from duplicate samples. Bars in plots represent median values. Comparisons across three genotypes were performed by using one-way analysis of variance.
