Fig. 2

PCR analysis of primates with duplicated marker TSp1. A: Gel electrophoresis results of PCR are shown. PCR conditions were as described15 except the annealing temperature was lowered to 50°C for cross-species amplification. Each lane is labeled to indicate the template DNA tested by PCR. K = KG1, a somatic cell hybrid that contains a single human chromosome 22 in a hamster x human hybrid, Hu = human, Ch = pygmy chimpanzee, Go = gorilla, Rh = rhesus monkey, Om = owl monkey, Ga = galago, and W = negative control with no template DNA. M = 1 kb DNA size marker. B: An evolutionary tree that shows the evolution and divergence of the different primate species used in the analysis in A. The approximate time of divergence of each species is indicated below each fork. mya = million years ago. The pygmy chimpanzee, gorilla, and rhesus monkey fibroblast cell lines were obtained from Coriell Mutant Cell repositories (Camden, NJ). The owl monkey and galago cell lines were kind gifts from Dr. Prescott Deininger.