Fig. 1
From: Gross BMPR2 gene rearrangements constitute a new cause for primary pulmonary hypertension
Southern blot analysis of genomic DNA from PPH patients and normal controls after digestion with restriction endonucleases EcoRI (A), HindIII (B), BamHI (C), or BglII (D) and hybridization with a 32P-labeled BMPR2 gene cDNA probe. Numerical listing for each lane corresponds to the Vanderbilt University Medical Center PPH cohort family number. Normal control DNA simultaneously analyzed for each restriction endonuclease digestion is denoted with a C. Normal control DNA demonstrating homozygosity for the loss of a BglII digestion site, as seen in PPH family 67, is noted by a C*. Arrows denote novel bands detected in PPH patients suggestive of BMPR2 gene rearrangements.
