Fig. 1 | Genetics in Medicine

Fig. 1

From: Bax gene G(-248)A promoter polymorphism is associated with increased lifespan of the neutrophils of patients with osteomyelitis

Fig. 1

Detection of the G(-248)A polymorphism in the bax gene. (Left) Genomic DNA of patients with OM was amplified, and PCR products were digested with the enzyme Aci I endonuclease followed by 7.5% polyacrylamide gel analysis. GG denotes a wild-type sample showing three distinct bands: 352, 296, and 96 bp; GA denotes a heterozygous sample showing the 352- and 296-bp bands and an almost invisible 96-bp band; AA denotes a homozygous SNP sample showing only the 35-bp band. MW, molecular weight marker, 100-bp DNA ladder (GeneRuler). (Right) Sequencing of the restriction fragment length polymorphism. Samples with no alteration: wild type (upper) with a heterozygous G-to-A replacement (middle) and a homozygous AA genotype (lower).

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