Fig 4

Confirmation of array comparative genomic hybridization (aCGH) copy number alterations (CNAs) by fluorescence in situ hybridization (FISH). (Top) A recurrent CNA from the lung adenocarcinoma study was observed on band 5p15.2, close to the cellular locus for the TRIO gene. The BAC, RP11-81P9, circled in red and adjacent to the TRIO gene coding region, was used to confirm CNAs by FISH (image, Roswell Park Cancer Institute aCGH viewer). (Bottom) FISH analysis for the TRIO gene (RP11-81P9; red signal) and a reference probe on the opposing chromosome arm (RP11-51D11; 5q35; green signal), the fraction of the population showing gains of the TRIO gene sequence by FISH was similar to the fraction estimated by aCGH log₂ values; left to right: interphase nuclei from the same tumor showing two, three, and four copies of the TRIO gene, respectively.