Fig 3
From: Comprehensive validation of array comparative genomic hybridization platforms: how much is enough?
Example of a poorly performing clone and the effect of low copy repeat (LCR) structure on the ability of array comparative genomic hybridization (CGH) to detect copy number changes. (A) Array CGH plot of sample 49 showing 16pter duplication. The terminal subtelomere clone RP11-568F1 does not demonstrate duplication. (B) Fluorescence in situ hybridization using clones RP11-568F1 (labeled in red) and GS-121-I4 (labeled in green) on 16pter to produce yellow fusion signals in cells from Patient 49. Arrows indicate fusion signals on the two normal chromosomes 16 and the derivative chromosome 8. Significant cross-hybridization of the RP11-568F1 (red) probe to multiple additional chromosomal locations in both metaphase and interphase was present. (C) UCSC Genome Browser representation of the genomic region covered by clone RP11-568F1. Brackets indicate LCR sequences present within this clone and on chromosomes 1, 2, 9, 15, 19, X, and Y.
