Fig. 1 | Genetics in Medicine

Fig. 1

From: Mitochondrial DNA analysis by multiplex denaturing high-performance liquid chromatography and selective sequencing in pediatric patients with cardiomyopathy

Fig. 1

Flowchart of the mDHPLC assay steps and sequencing analysis. The mitochondrial genome was amplified in 19 overlapping sections according to the Mitoscreen Assay protocol (Transgenomic). The majority of amplicons were digested with restriction enzymes and analyzed at multiple temperatures. Variant peak profiles were further investigated by DNA sequencing. *One representative fragment was typically sequenced in cases of highly similar variant DHPLC patterns.

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