Fig. 2

C. elegans mitochondrial stability study. The RCC was measured by the Hahn laboratory after multiple cycles of freeze-thaw over time. The panel on the right summarizes the number of cycles of freeze-thaws and length of storage. Complex IV activity was missing in Sample J due to spectrophotometer technical issue. Complex IV activity marginally declined over time with successive cycles of freeze-thaw but not at a magnitude that would affect interpretation, when compared with mutants. Overall, the entire RCC activities remained stable over 3 months after up to four freeze and thaws. CS, citrate synthase; I + III, Complexes I + III; I, Complex I; II + III, Complexes II + III; II, Complex II; III, Complex III; IV, Complex IV.