Figure 3
Gene transduction efficiency of various organs and subcutaneous tumors following intravenous injection of adenoviruses. The mice were injected intravenously with 2 × 109 PFU of adenoviruses (AdΔCAR, AdΔCAR-SYE or Ad-EGFP; n=3), and then 5 μm slices of frozen sections at day 6 were examined under fluorescence microscopy ( × 200 original magnification). (a) Gene transduction efficiency of various organs. Left: representative photographs of the liver and spleen ( × 200 original magnification). Right: number of enhanced green fluorescent protein (EGFP)-positive foci. EGFP-positive foci were counted in at least four fields at day 6 in various organs such as the lung, kidney, pancreas, spleen and liver, and mean±s.d. are presented. (b) Gene transduction efficiency of subcutaneous tumors. Left: representative photographs of the AsPC-1 and PC3 tumors ( × 200 original magnification). Right: number of EGFP-positive foci. EGFP-positive foci were counted in at least four fields at day 6 in tumors, and mean±s.d. are presented.
