Figure 4
In vitro cell killing by infection of AdΔCAR-SYE. (a) Crystal violet staining of viable cells 7 days after infection. AsPC-1 and PC3 cells were seeded at 1 × 105 cells per well in 24-well plates and 24 h later the cells were infected with AdΔE1, AdΔCAR, AdΔCAR-SYE or Ad-EGFP at multiplicities of infection (MOIs) of 0.1, 1, 10 and 100. Seven days after the infection the cells were fixed with 4% paraformaldehyde for 10 min and stained with 1% crystal violet staining solution. (b) Cell killing by adenoviruses in various cell lines. The cells were infected with AdΔCAR, AdΔCAR-SYE or Ad-EGFP at MOIs of 5, 10, 30, 50 and 100, and cell growth was determined by the in vitro cell proliferation assay 7 days after the infection. The assays (carried out in eight wells) were repeated a minimum of two times, and mean±s.d. are presented. The statistical differences between OD450 values of cells infected with AdΔCAR-SYE and those with AdΔCAR at MOI of 30 are presented.
