Figure 5
Tumor growth suppression after intratumoral injection of AdΔCAR-SYE. (a) Tumor growth of AsPC-1 and PC3 subcutaneous tumors. The 0.5∼5 × 108 PFU of adenoviruses (AdΔE1, AdΔCAR, AdΔCAR-SYE or Ad-EGFP) was directly injected once into the subcutaneous tumors (n=6∼7). Tumor volume was monitored, and mean±s.d. is presented on the days indicated. The statistical differences between tumor volumes infected with AdΔCAR-SYE and those with AdΔCAR are presented. (b) Enhanced green fluorescent protein (EGFP) expression in AsPC-1 subcutaneous tumors. The intratumoral replication of adenoviruses (AdΔE1-EGFP, AdΔCAR or AdΔCAR-SYE) was assessed in the tumors. Frozen sections of tumors injected with each virus were examined at days 2, 4 and 6 under fluorescence microscope ( × 200 original magnification). (c) Immunodetection of adenoviral hexon proteins in the treated tumors. Six days after the intratumoral injection of adenoviruses (AdΔE1, AdΔCAR or AdΔCAR-SYE), the frozen sections of tumors were stained with anti-hexon antibody ( × 200 original magnification). (d) Increase of virus titer in the AsPC-1 tumors. 1 × 108 PFU of AdΔCAR or AdΔCAR-SYE was injected into the AsPC-1 subcutaneous tumors (n=3), which were collected 4 and 6 days after the virus injection. The 293-38.HissFv.rec cells were infected with the crude viral lysate of the tumors and 24 h after the infection EGFP-expressing cells were counted under florescence microscope to assess the titer of infectious virus. Titer of virus per tumor is presented.
