Figure 6 | Gene Therapy

Figure 6

From: Oncolytic virus therapy for pancreatic cancer using the adenovirus library displaying random peptides on the fiber knob

Figure 6

Ectopic infection of normal organs after intratumoral injection of adenoviruses. (a) Detection of adenovirus DNA by PCR method. AsPC-1 subcutaneous tumors and organs (the liver, spleen and lung) were collected 2 and 6 days after the virus injection (AdΔE1, AdΔCAR, AdΔCAR-SYE or Ad-EGFP), and DNA from tissues was subjected to PCR with the primers of coxsackievirus and adenovirus receptor (CAR) binding domain in adenovirus fiber knob. (b) Enhanced green fluorescent protein (EGFP)-positive cells in the liver. EGFP-positive cells were examined in frozen sections of the liver under fluorescence microscope 6 days after the direct injection of adenoviruses (AdΔE1-EGFP, AdΔCAR, AdΔCAR-SYE or Ad-EGFP) into AsPC-1 tumors ( × 200 original magnification). (Arrows: EGFP-positive cells.)

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