Figure 5
In vivo tumor treatment experiments in mice that received a high therapeutic dose of noncarrier naked or gold particle-coated DNA vaccine. (a) Representative figures of pulmonary tumor nodules in each group: 1, naïve; 2, no insert; 3, E7; 4, naked CRT/E7 twice; 5, naked CRT/E7 thrice; 6, naked CRT/E7 four times; 7, gold particle-coated CRT/E7. (b) Mean pulmonary tumor nodules in mice vaccinated with different frequencies of noncarrier naked CTGFCRT/E7 DNA vaccine and gold particle-coated CRT/E7 DNA vaccines (note: mice that received the noncarrier naked CRT/E7 DNA vaccine had less pulmonary tumor nodules than those that received the noncarrier naked wild-type E7 DNA vaccine). Mice that were treated with noncarrier naked CRT/E7 DNA four times showed significantly less pulmonary tumor nodules than those that were treated twice or thrice. The number of pulmonary tumor nodules was not different between mice immunized with noncarrier naked and gold particle-coated CRT/E7 DNA vaccine four times. (c) Mean pulmonary tumor nodules in mice vaccinated with noncarrier naked CRT/E7 DNA in various media (note: the mean pulmonary tumor nodules of mice vaccinated with noncarrier naked CRT/E7 DNA in distilled H2O (ddH2O) or Tris-EDTA (TE) solution was significantly lower than those in mice treated with noncarrier naked DNA vaccine in phosphate-buffered saline (PBS).
