Figure 6 | Gene Therapy

Figure 6

From: Noncarrier naked antigen-specific DNA vaccine generates potent antigen-specific immunologic responses and antitumor effects

Figure 6

Activation of E7-specific CD8+ T cells by CD11c-enriched cells isolated from the inguinal lymph nodes of noncarrier naked or gold particle-coated DNA vaccinated mice. (a) Representative figures of flow cytometry analysis. (b) Numbers of interferon (IFN)-γ-secreting E7-specific CD8+ T cells stimulated by CD11c-enriched cells isolated from the inguinal lymph nodes of vaccinated mice (note: CD11c-enriched cells isolated from mice vaccinated with noncarrier naked and gold particle-coated CRT/E7 DNA vaccines were more effective in activating the E7-specific CD8+ T-cell line to secrete IFN-γ compared with noncarrier naked wild-type E7 DNA vaccine). However, there was no difference in the numbers of IFN-γ-secreting CD8+ T cells between gold particle-coated and noncarrier naked CRT/E7 DNA vaccines in respective solution media. The data collected from all of the above experiments are from one representative experiment of the two experiments that were carried.

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