Figure 7
Burning effects on the skin of noncarrier naked and gold particle-coated DNA vaccinated mice. Mice were first given noncarrier naked CRT/E7 chimeric DNA or gold particle-coated CRT/E7 DNA at 50 psi as described in the Materials and methods section. (a) Shaved skin before vaccination. (b) Mice that received noncarrier naked DNA vaccine immediately. (c) Mice that received gold particle-coated DNA vaccine immediately. (d) Mice that received noncarrier naked DNA vaccine 24 h later. (e) Mice that received gold particle-coated CRT/E7 DNA vaccine 24 h later. (f) Mice that received noncarrier naked DNA vaccine after 3 days. (g) Mice that received gold particle-coated DNA vaccine after 3 days. (h) Mice that received noncarrier naked CRT/E7 DNA vaccine after 7 days. (i) Mice that received gold particle-coated CRT/E7 DNA vaccine after 7 days (note: the skin damage was noted from 24 h after being given the gold particle-coated DNA vaccine and the damage continued until 7 days postimmunization. There was no visible skin damage for mice that received noncarrier naked DNA immunization.
