Figure 1 | Gene Therapy

Figure 1

From: Down-modulation of cancer targets using locked nucleic acid (LNA)-based antisense oligonucleotides without transfection

Figure 1

Down-modulation of target mRNA with LNA–ONs in the absence of lipofection in 15PC3 cells. LNA–ONs were added to tissue culture media at the indicated concentrations and incubated with 15PC3 cells (plated at approximately 2000 cm–2). Down-modulation of HER3 (a), β-catenin (b), PIK3CA (c) and HSP27 (d) mRNA after treatment for 3 (a, b) or 6 days (c, d) were determined by quantitative real-time PCR with TaqMan gene expression assay. LNA–ONs designed to specifically targets are HER3 (EZN-3920), HSP27 (EZN-4516), PIK3CA (EZN-4150) or β-catenin (EZN-3889). Data are shown as means±s.e. (n=3).

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