Figure 2 | Gene Therapy

Figure 2

From: Down-modulation of cancer targets using locked nucleic acid (LNA)-based antisense oligonucleotides without transfection

Figure 2

Kinetics of target mRNA down-modulation with naked LNA–ONs. (a, b) 15PC3 was plated at approximately 2,000 cm–2 in six-well plates and cultured for 5 days. At different time points, 5 μM of either EZN-3920 or EZN-4516 were added to designated wells to start the treatment. At the end of day 5, the cells were harvested and total RNA extracted for determining the time course of HER3 (a) or HSP27 (b) mRNA levels. (c) PC3 cells were similarly treated with 5 μM of either EZN-4150 or EZN-3920 and measured for PIK3CA mRNA levels. (d) SW480 were treated with 5 μM of EZN-3889 and measured for β-catenin mRNA levels at different time points. Data are shown as means±s.e. (n=3). The specificity of EZN-3889-mediated down-modulation of β-catenin mRNA in SW480 is shown in Supplementary Figure S2.

Back to article page