Figure 3 | Gene Therapy

Figure 3

From: Down-modulation of cancer targets using locked nucleic acid (LNA)-based antisense oligonucleotides without transfection

Figure 3

Nuclear localization of LNA–ONs correlated with mRNA down-modulation. (a) 15PC3 cells were treated with FAM-labeled EZN-3920 for the designated time and examined using fluorescence microscopy for FAM and Hoechst signals. Cells with apparent nuclear staining of FAM-EZN-3920 are indicated with arrows. (b) Comparison of FAM and Hoechst signals detected in 15PC3 and A549 cells after cells were incubated in the media containing 2 μM FAM-labeled EZN-3920 for 24 h. (c) 15PC3 and A549 were treated with 1 and 2 μM EZN-3920, respectively. HER3 mRNA levels were examined after 24 h. Error bars indicate s.d. (n=3).

Back to article page