Figure 4 | Gene Therapy

Figure 4

From: Down-modulation of cancer targets using locked nucleic acid (LNA)-based antisense oligonucleotides without transfection

Figure 4

Efficient protein down-modulation by LNA–ONs. (a) 15PC3 cells were treated with 1 or 5 μM EZN-3920 and analyzed for HER3 protein levels by immunoblotting on day 1 or day 5. β-catenin and tubulin were also analyzed for specificity. (b) 15PC3 cells were treated with indicated concentrations of EZN-4516. HSP27 protein levels were analyzed on day 1, 3 or 5. HER3 and β-tubulin proteins were also probed to indicate specificity. (c) 15PC3 cells were treated with indicated concentrations of EZN-4150 for 6 days. PIK3CA, p-AKT, AKT and HSP27 proteins were analyzed by immunoblotting. (d) SW480 cells were treated with indicated concentrations of EZN-3889 (duplicates) for 6 days and analyzed for β-catenin or β-tubulin protein levels by immunoblotting.

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