Figure 5
Effect of LNA–ONs on the growth of cancer cells. (a) Multiple cell lines were plated at approximately 1000 cm–2 and treated with 2.5 μM EZN-3920. MTS (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed on day 7 to show the effect of compound on the growth of cells. Data are means±s.e. (n=8); (b, c) 15PC3 or HCC827 cells were treated with 0.25–5 μM EZN-3920 or indicated control LNA–ONs for 6–8 days. The control ONs were a scrambled ON (EZN-3046) and three base pair mismatched compared with EZN-3920 (EZN-4454, 4455). Data are means±s.e. (n=3).
