Table 1 Characterization of vector copies and vector insertion sites in HT1080 clones

From: Quantification of lentiviral vector copy numbers in individual hematopoietic colony-forming cells shows vector dose-dependent effects on the frequency and level of transduction

Clones

VCN by Q-PCR (Taqman)

Insertion site analysis by VITA (n=2)

 

n

VCN Ave±s.d. (extremes)

Nb IS

Size(bp)_Chrom(no.)_Position

Gene

Description function

HT4-A

37

1.1±0.2 (0.74–1.5)

1

107_19_13093976

BTBD14B

Transcriptional regulator

    

285_8_81041615

MRPS28

Mitochondrial ribosomal protein

HT4-A2

16

2.79±0.65 (1.9–3.6)

3

183_14_71097682

SIPA1L1

Signal-induced proliferation-associated protein

    

107_19_13093976

BTBD14B

Transcriptional regulator

    

164_3_178970571

No gene

 
    

136_17_10245972

MYH8

Myosin heavy chain 8, skeletal muscle

    

114_17_44346886

UBE2Z

Ubiquitin-conjugating enzyme E2Z

HT4-A6

17

7.13±1.32 (5.3–8.7)

8

330_17_77173163

NPLOC 4

Nuclear protein localisation 4

    

63_12_61459846

PPM 1H

Protein phosphatase 1H

    

68_7_19590033

No gene

 
    

62_7_101494262

CUX-1

Cut-like homeobox 1a

    

107_19_13093976

BTBD14B

Transcriptional regulator

  1. Abbreviations: Q-PCR, quantitative-PCR; s.d., standard deviation; VCN, vector copy numbers; n, number of Q-PCR experiments.
  2. The bold data represent the identical insertion that is expected to be found in the three different cell lines.
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