Figure 3
Mice treated with bPΔ6-hPAP show tumor reduction and increased survival. C57/BL6 mice were implanted subcutaneously in the flanks with TRAMP-C2 cells (5 × 106 per mouse). Once tumors were established (50–100 mm3) mice (N=8) were randomized and treated four times with 1 × 107 PFU of bPΔ6-empty, bPΔ6-mPAP, bPΔ6-hPAP or phosphate-buffered saline (PBS) over a period of 10 days by direct intra-tumoral inoculation. Tumor volumes were monitored using calipers and calculated with the formula v=[(length) × (width2)]/2. Mice were killed when tumors reached 1000 mm3. Animals treated with bPΔ6-hPAP showed a significant reduction in tumor volume (P=0.002; PBS vs hPAP, P=0.04; empty vs hPAP, P=0.03; mPAP vs hPAP) (a) and a significant increase in survival (b) when compared with the bPΔ6-mPAP (P=0.01), bPΔ6-empty (0.01) or PBS (P=0.0008) groups. Analysis of tumor volumes (day 24, Student's t-test) and survival (log-rank test) was performed with GraphPad Prism v.4 (San Diego, CA, USA).
