Figure 3 | Gene Therapy

Figure 3

From: Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

Figure 3

Effect of the 5′ cap on half-life and stability of mRNAs in DCs. (a) Human immature and (b) mature DCs (iDCs and mDCs, respectively) were electroporated with equal amounts of d2eGFP-encoding mRNAs capped with different cap analogs. Cells were harvested after 2, 4, 8, 24, 48 and 72 h, RNA isolated from the cells, and the d2eGFP-transcript levels were quantified by real-time reverse transcriptase-PCR (RT-PCR). For each time point, the difference between the threshold cycles (Ct) of RNAs encoding d2eGFP and hypoxanthine phosphoribosyltransferase (HPRT1) used as internal control was calculated. The data were fitted to a biphasic (immature DCs), or to a monophasic decay curve (mature DCs). The mRNA half-lifes calculated from the data shown here are listed in Table 2.

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