Figure 4 | Gene Therapy

Figure 4

From: Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

Figure 4

Competition for translation between ARCA- and β-S-ARCA(D1)-capped RNAs. Protein decay kinetics in human immature DCs were determined by measuring luciferase activities at different time points after electroporation with the indicated RNA species. (a) Ratios of luciferase activities measured at the respective time points in cells electroporated with either 40 or 20 pmol of RNAs equipped with the same cap (±s.d. of two independent experiments). (b) Cells were electroporated with luciferase RNA alone or together with d2eGFP RNA as competitor (comp.) each equipped with the indicated cap. Luciferase activity was recorded and set in relation to luciferase activity of cells electroporated exclusively with the respective luciferase species without competitor to obtain the ‘relative signal’ (set to 1 for both ARCA- and β-S-ARCA(D1)-capped RNA).

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