Figure 5 | Gene Therapy

Figure 5

From: Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

Figure 5

Effect of the 5′ cap on protein expression in vivo. Two groups of mice (each n=9) received injections of equal amounts of luciferase-encoding mRNA with either ARCA or β-S-ARCA(D1) 5′ cap structures into inguinal lymph nodes. In vivo imaging of luciferase activity (given in pps) was performed at different time points. (a) Imaging series of representative mice. The photon counts are represented as given by the color scale at the bottom. (b) Time kinetics of average luciferase activity. Error bars indicate standard error of the mean (s.e.m.). Statistical analysis (Student's t-test) was performed for the measurements after 8 and 24 h (*P<0.005 and **P<0.02). (c) Time kinetics of luciferase activities for all mice. For each cap analog, the curves were normalized based on the total protein expression to better visualize the functional RNA stability.

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