Figure 6 | Gene Therapy

Figure 6

From: Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

Figure 6

Effect of the 5′ mRNA cap structure on de novo priming of naïve T cells after intranodal immunization with antigen-encoding mRNA. Two groups of mice (each n=5) were intranodally immunized twice (day 0 and day 3) with equal amounts of SIINFEKL-encoding mRNA capped either with ARCA or β-S-ARCA(D1). The frequency of SIINFEKL-specific CD8+ T cells was quantified by tetramer staining on day 8. As a control, one group of mice (n=3) was treated with buffer (no RNA) (a) Representative dot-plots of cells from peripheral blood and spleen of immunized mice are shown. (b) Average number of tetramer positive CD8+ cells (in %) as measured on day 8. Error bars indicate standard error of the mean (s.e.m.). Statistical analysis (Student's t-test) was performed (*P<0.075).

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