Table 1 Effect of the 5′ mRNA cap structure on translational properties
5′ Cap structure | Cells | Transl. efficiency | Time of max. (hours) | Total protein expression |
|---|---|---|---|---|
No cap | iDCs | 0.00±0.00 | NA | 0.011±0.000 |
ApppG | iDCs | 0.01±0.00 | NA | 0.022±0.001 |
GpppG | iDCs | 0.18±0.01 | 11.5±0.0 | 0.221±0.001 |
m7GpppG/c.t. | iDCs | 1.00±0.04 | 8.1±1.1 | 1.000±0.007 |
m7Gppppm7G | iDCs | 0.20±0.10 | 4.9±0.1 | 0.404±0.004 |
ARCA | iDCs | 2.52±0.19 | 12.6±0.1 | 4.777±0.042 |
β-S-ARCA(D1) | iDCs | 3.36±0.09 | 15.4±0.1 | 13.094±0.307 |
β-S-ARCA(D2) | iDCs | 3.53±0.17 | 12.8±0.0 | 6.570±0.075 |
m7GpppG/p.t. | iDCs | 4.12±0.53 | 8.4±1.4 | 4.289±0.056 |
No cap | mDCs | 0.00±0.00 | NA | 0.002±0.000 |
ApppG | mDCs | 0.01±0.00 | NA | 0.008±0.000 |
GpppG | mDCs | 0.31±0,02 | 27.5±0.5 | 0.593±0.005 |
m7GpppG/c.t. | mDCs | 1.00±0.05 | 16.0±0.3 | 1.000±0.003 |
m7Gppppm7G | mDCs | 0.56±0.04 | 6.0±0.3 | 0.176±0.001 |
ARCA | mDCs | 2.16±0.00 | 17.5±0.3 | 2.526±0.015 |
β-S-ARCA(D1) | mDCs | 3.05±0.14 | 20.1±0.1 | 3.884±0.032 |
β-S-ARCA(D2) | mDCs | 3.30±0.03 | 19.4±0.1 | 4.042±0.053 |
m7GpppG/p.t. | mDCs | 3.16±0.08 | 17.5±0.3 | 3.421±0.010 |
