Figure 4

Iodine efflux from K1 and F133 cells infected with AdPax-8. (a) K1 cells were infected with 20 MOI AdCMV or 20 MOI AdPax-8 for 48 h. (b) F133 cells were infected with 20 MOI AdCMV or 20 MOI AdPax-8 for 48 h. Thereafter, the two kinds of cells were incubated in medium containing 3.7 KBq Na¹²⁵I for 1 h. The medium containing ¹²⁵I was replaced with fresh non-radioactive medium every 5 min, and in the replaced medium radioactivity was measured. After the last medium was removed, the cells were extracted. The total radioactivity present at the initiation of the efflux study (100%) was calculated by adding the counts in the final tissue extract to the medium counts. Data are expressed as the mean±s.e.m. (n=6).