Figure 1

Comparison of expression durations mediated by different systems. (a) Schematic illustrations of the plasmids. (b) Gene expression profiles. HEK293 cells were transfected with equal amounts of the plasmids, continued to be cultured, passaged and analyzed by flow cytometry. The total FIs were yielded by multiplying the number of GFP+ cells and mean FI, and are expressed in a.u. The data represent the average total FI of three independent culture experiments.