Figure 3 | Gene Therapy

Figure 3

From: Development of the hybrid Sleeping Beauty-baculovirus vector for sustained gene expression and cancer therapy

Figure 3

The hybrid SB-baculovirus Bac-SB-T2hEA/w prolonged the hEA expression. (a) Schematic illustration of the baculovirus constructs. (b) hEA expression profile. HEK293 cells were transduced with Bac-T2hEA/w (deficient in SB expression) or Bac-SB-T2hEA/w and continued to be subcultured every 3 days. The supernatant was collected at different times for hEA expression analyses. W denotes WPRE, a cis-acting element that enhanced the baculovirus-mediated transgene expression in vertebrate cells.

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