Table 6 Analysis of mRNA expression levels and of lipid peroxidation in the remote LV myocardium in non-infarcted control C57BL/6 LDLr−/− mice and in control and AdLDLr-treated C57BL/6 LDLr−/− mice 28 days after MI

From: Low-density lipoprotein receptor gene transfer in hypercholesterolemic mice improves cardiac function after myocardial infarction

 

Non-infarcted

28 Days after MI

 

Control

Control

AdLDLr

Gene expression (mRNA expression normalized to Gapdh)

 Agtr1a

1.0±0.098

0.43±0.018§§§

0.58±0.033***§§

 Ctgf

1.0±0.15

9.3±1.1§§§§

5.2±0.96*§§§

 Nox2

1.0±0.12

2.0±0.21§§

1.5±0.22

 Nox4

1.0±0.19

9.0±1.2§§§§

4.8±0.95*§§§

 Nppa

1.0±0.29

10±1.3§§§§

11±3.1§§§

 Nppb

1.0±0.22

4.8±0.31§§§§

2.6±0.42***§§

 Sod1

1.0±0.10

1.2±0.070

1.0±0.12

 Sod2

1.0±0.10

0.96±0.078

1.0±0.079

 Sod3

1.0±0.31

1.7±0.20

1.6±0.31

Lipid peroxidation

 MDA (nmol mg protein–1)

2.5±0.19

2.2±0.17

2.7±0.17

  1. Abbreviations: AdLDLr, adenoviral LDLr; Agtr1a, angiotensin II receptor, type 1a; Ctgf, connective tissue growth factor; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; MDA, malondialdehyde; MI, myocardial infarction; Nox, NADPH oxidase; Nppa, natriuretic peptide precursor type A; Nppb, natriuretic peptide precursor type B; LDLr, LDL receptor; LV, left ventricular; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction Sod, superoxide dismutase; TBARS, thiobarbituric acid reactive substances.
  2. *P<0.05, ***P<0.001 for comparison of control versus AdLDLr-treated mice.
  3. §§P<0.01, §§§P<0.001, §§§§P<0.0001 for comparison versus non-infarcted mice.
  4. Data are presented as means±s.e.m. qRT-PCR analysis (n=7–15 per group) was performed using Taqman gene expression assays. Gene expression data analysis was performed using ΔΔCt-based fold-change calculations and data are expressed relative to values in non-infarcted control mice. The degree of lipid peroxidation in LV homogenates (n=8 per group) is expressed as MDA equivalents (nmol) per mg protein using the TBARS assay according to the instructions of the manufacturer (Cayman Chemical).