Figure 6
From: Bacterial magnetic particles as a novel and efficient gene vaccine delivery system
The anti-tumour effects of different amounts of BMP-V. C57BL/6 mice inoculated s.c. with 5 × 104 TC-1 cells. When tumours reached an average diameter of ∼5 mm on day 4, mice were randomised and treated with different doses of BMP-V (40, 20, 10, 5 μg of BMP-V in 0.1 ml PBS or PBS alone). The same treatments were repeated on days 8 and 12. BMP-V were delivered to mice in the shaved inguinal region through subcutaneous injection with a magnet (magnetic flux density of 600 mT) on the injection site for 10 min. (a) Tumour volumes were monitored for 25 days until the control mice began to die. Data are presented as mean tumour volumes±s.d. of five mice per group in a representative experiment. *Indicates P<0.05, all treatments compared with PBS. (b) Survival of mice per treatment group. The results of both experiments were pooled in a stratified analysis, resulting in P>0.05 when the 5 μg BMP-V-treated group was compared with the 10 μg BMP-V-treated group and P<0.05 when the 5 μg BMP-V-treated group was compared with the PBS group. Data are representative of three independent experiments. MST: mean survival time.
