Figure 5
From: AAV-mediated in vivo knockdown of luciferase using combinatorial RNAi and U1i
AAV-mediated in vivo knockdown of luciferase using RNAi and U1i. Murine muscle was co-transduced with 1 × 1010 gc AAV-Luc and 4.5 × 1010 gc AAV-shRNA/U1i per hind limb. The gastrocnemic and adductor muscles of both hind limbs were injected. Six mice per treatment were injected, resulting in n=12. Luciferase expression was measured in the IVIS at 1, 2, 3, 4, 6, and 8 weeks post-transduction. The control vector AAV-AH expressed shApoB and double H1/H4. The U1i vector AAV-AL expressed shApoB and double L4/L5. The shRNA vector AAV-LH expressed shLuc1 and double H1/H4. The shRNA and U1i combination vector AAV-LL expressed shLuc1 and double L4/L5, and this viral vector significantly reduced luciferase expression on average by 25% during the course of the experiment (P<0.05). Data are presented as mean (n=12)±s.e.
