Figure 2

Doxycycline treatment enhances protein level and surface translocation of MICA/MICB, and enhances phosphorylated ATM levels. (a) Overall protein levels of MICA/B was detected in UCI-101 and OVCAR4 cell lines 24 h after treatment with the indicated concentrations of doxycycline by Western Blotting. (b) Immunofluorescence confocal microscopy was used to detect the subcellular location of MICA/B (green) after treatment with 40 μgml⁻¹ doxycycline for 24 h in UCI-101 and OVCAR4 cell lines. Phalloidin is red and Draq-5 (nuclei) are blue. 40 × magnification. (c) Levels of phosphorylated ATM and overall ATM was determined in the indicated cell lines at the indicated times after treatment with 1 μgml⁻¹ doxycycline, as detected by western Blotting.