Figure 5 | Gene Therapy

Figure 5

From: Inhibition of neointimal hyperplasia in a rabbit vein graft model following non-viral transfection with human iNOS cDNA

Figure 5

Double immunofluorescent characterisation of iNOS-positive cells in 7-day vein graft samples. Immunostaining using first-layer antibodies is shown in green for iNOS (a–c). Second-layer antibodies were labelled in red for CD31 on endothelial cells (a), RAM11 for macrophages (b) and HHF35 for smooth muscle actin (c). Overlapping staining was revealed by yellow fluorescence. DAPI staining (blue) was performed to detect cell nuclei (a–c). L, lumen. Bars=46.54 μm in (a) and (b), =47.62 μm in (c).

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