Figure 4 | Gene Therapy

Figure 4

From: Virally expressed connexin26 restores gap junction function in the cochlea of conditional Gjb2 knockout mice

Figure 4

Results of FRAP assay for evaluating the intercellular coupling among the cochlear cells of WT, treated and untreated cCx26KO mice. (a) Time series images showing the fluorescence recovery in the photobleached cells (indicated by white arrows) of WT (top row), treated (middle row) and untreated (bottom row) cCx26KO mouse cochlea. Time interval of each panel is 1 min. The first panel shows the outer sulcus cells loaded with calcein AM just before photobleaching. The cell pointed by the white arrow was photobleached by intense laser light for 10 s, after which the fluorescence recovery in the cell is measured. (b) Quantification of the FRAP results showing the relative fluorescence intensity change around the time when the cell is photobleached.

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