Figure 4 | Gene Therapy

Figure 4

From: CD40 ligand and tdTomato-armed vaccinia virus for induction of antitumor immune response and tumor imaging

Figure 4

In vitro and in vivo expression of hCD40L. (a) A549 cells were infected with different p.f.u. per cell of vvdd-hCD40L. Supernatant was collected at different time points and analyzed by FACSarray for expression of hCD40L. Nude mice bearing M4A4-LM3 breast tumors were intratumorally injected with vvdd-tdTomato or vvdd-hCD40L-tdTomato-tdTomato. (b) Mice were imaged by IVIS. (c) Blood samples were collected on days 3 and 13, and tumors were collected on day 13. Blood and tumors were analyzed for hCD40l concentration with FACSarray.

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