Figure 4
AdLCY exhibited higher tumor-selective cytolytic effect than Ad9OC on bladder cancer cells. (a) Analysis of surface expression of CAR on bladder cancer and normal epithelial cells by flow cytometry. Black lines denote cell percentages expressing CAR. Red zones represent background fluorescence on the cells. (b) Detection of AdGFP-infected cells. Various cells were infected with AdGFP at an MOI of 10 or mock-infected for 48 h. Cells expressing GFP were observed under fluorescence microscopy. Representative images in each group are shown ( × 40 magnification; scale bar=500 μm). (c) Cytotoxic effects of AdLCY and Ad9OC. Bladder cancer and normal epithelial cells were infected with increasing MOIs of AdLCY or Ad9OC, or were mock-infected under normoxia (N) or hypoxia (H) for 48 h. Cells were then cultured in normoxic conditions for 5 days, and cell survival was determined by the WST-1 assay. Values are the mean±s.e.m. of the mean. ***P<0.001; **P<0.01; *P<0.05.
